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Fructose-6-Phosphate Kinase

Fructose-6-Phosphate Kinase

Cat no: F8060-05A

Supplier: United States Biological
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Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:6000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F8060-05A
Reactivities: Rabbit
Hosts: Goat
Applications: ELISA
Size: 2ml
Form: Supplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with 2ml of deionized water.
P type: Pab
Purity: Purified by delipidization and defibrination.
Additional info: Recognizes purified and partially purified Fructose-6-Phosphate kinase (Rabbit Muscle).

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