FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases.
Applications:
Suitable for use in Peptide ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions:
Peptide ELISA: 1:4,000.
Western Blot: 0.03-0.1ug/ml. A band at ~22kD band was observed in Human Brain (Cerebellum), Liver and Placenta lysates.
Immunohistochemistry (Paraffin): Shows strong staining of PCT cytoplasm, but not in DCT cytoplasm in human kidney.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.