FUT11 (Fucosyltransferase 11; also FucTXI and alpha 1,3 fucosyltransferase 11) is a 56kD (predicted) member of the glycosyltransferase 10 family of enzymes. It is potentially an ER associated enzyme that catalyzes the addition of alpha-L-fucose to a biantennary N-glycan acceptor. Rather than serve as a permanent modification, this fucosylation may serve as a quality control mechanism, flagging inappropriately glycosylated molecules. Human FUT11 is a type II transmembrane protein 492aa in length. It contains a short cytoplasmic region (aa1-7) plus an extended lumenal domain (aa25-492). that possesses a transferase region (aa60-360). There are two potential splice variants. One shows an Arg sunstitution for aa445-472, while a second shows a 31aa substitution for aa446-472. Over aa25-492, human FUT11 shares 88% aa identity with mouse FUT11.
Applications:
Suitable for use in ELISA, Immunoprecicpitation, and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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