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Fucosyltransferase 3 (FUT3, Galactoside 3(4)-L-fucosyltransferase, Blood Group Lewis alpha-4-Fucosyltransferase, Lewis FT)

Fucosyltransferase 3 (FUT3, Galactoside 3(4)-L-fucosyltransferase, Blood Group Lewis alpha-4-Fucosyltransferase, Lewis FT)

Cat no: F9200-48

Supplier: United States Biological
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Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha-4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa1-15), a transmembrane region (aa16-34) and an ectodomain (aa35-361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~20ng/lane under non-reducing and reducing conditions. Immunoprecipitation: 25ug/ml immunoprecipitates rhFUT3 or rhFUT5 from conditioned cell culture medium. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F9200-48
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human Fucosyltransferase 3 (FUT3) and human Fucosyltransferase 5 (FUT5). Species Sequence Homology: Chimapanzee FUT5, 98%.

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