Fumonisin, BioAssay(TM) ELISA Kit

Fumonisin B1 and B2 are mycotoxins, which are produced by Fusarium moniliforme, F. solani and other types of fusarium. These toxins show an extreme stability against high temperatures (up to 100 degrees C), and they can remain active in contaminated food for years. Fumonisin can be found in maize, oats and other types of grain. Worldwide a contamination in maize of 60% has been detected. When ingested by animals, fumonisin leads to neurotoxicity, hepatotoxicity and lung edema, mainly in horses and pigs. Therapeutic measures are the change of the grain given to the animals, or the administration of diuretic drugs. In human patients hints for the appearance of esophagus cancer could be associated with the exposition to fumonisin B1. Values assessed for the acute toxicity are 8 mg per kg weight and for the chronic situation 25mg/kg in feed stuff. These are relatively high concentrations, but according to the Good Manufacturing Practice, the content of fumonisin B1 in food intended for humans should not exceed 1mg/kg. The usual methods for detection of fumonisin in maize are liquid and thin-layer chromatography. This ELISA kit is very sensitive and allows the detection of trace amounts below 25ug per kg.\n\nSample Type:\nMaize and grain products.\n\nIntended Use:\nFor the quantitative determination of Fumonisin in maize and grain products.\n\nSensitivity: \n0.5ng/ml\n\nRecovery (spiked samples): ~80%\n\nIncubation Time: 80min\n\nTest Principle:\nThe United States Biological Fumonisin quantitative test is based on the principle of the enzyme linked immunosorbent assay. A microtiter plate is coated with antibodies raised against mouse immunglobulins. The standards and samples respectively are pipetted together with a fumonisin- peroxidase conjugate and a mouse-anti-fumonisin antibody into the appropriate wells. The conjugate competes with the fumonisin of samples/standards for the limited number of antibody sites. Simultaneously the anti-fumonisin antibody is bound to the anti-mouse antibody coated on the mi- crotiter plate. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of fumonisin is indirectly proportional to the color intensity of the test sample.\n\nKit Components:\n1. Microtiter plate 1x96 wells each, coated with anti-mouse immunglobulin.\n2. Standard 0ng/ml 1x1ml, ready to use.\n3. Standard 0.5ng/ml 1x1ml, ready to use.\n4. Standard 2ng/ml 1x1ml, ready to use.\n5. Standard 5ng/ml 1x1ml, ready to use.\n6. Standard 10ng/ml 1x1ml, ready to use.\n7. Standard 25ng/ml 1x1ml, ready to use.\n8. Conjugate (Fumonisin-Peroxidase): 1x6ml, ready to use.\n9. Anti-Fumonisin Antibody: 1x6ml, ready to use.\n10. Substrate Solution (TMB): 1x15ml, ready to use.\n11. Stop Solution (0.5M H2SO4): 1x15ml, ready to use.\n12. Sample Diluent (PBS): 2x60ml, ready to use.\n13. Washing Solution 10x (PBS + Tween 20): 1x60ml. \nDilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37 degrees C for 15 minutes.\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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