Furin Convertase

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10kD amino-terminal proregion, a highly conserved ~55kD subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface. \n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000; Detects an ~100kD protein representing Furin in BSC-40 cells transfected with the human Furin gene. Detects human Furin transfected into 3T3 cells.\nImmunoprecipitation: 1:100\nImmunofluorescence: Staining of endogenous Furin convertase in 3T3 cells yields a pattern consistent with paranuclear staining.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F9201

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Can

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

General: \nThis antibody has been used in:J. Biol. Chem. 276:35953-35960 (2001).J. Cell Biol. 139:1719-1733 (1997).General:EMBO J., 13: 18-33, 1994.J. Biol. Chem., 267: 23435-23438, 1992.J. Cell Biol., 111: 2851-2859, 1990.J. Histochem. & Cytochem., 45: 3-12, 1997.

Additional Info

Recognizes Furin convertase in transfected human Furin. Does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue. Species Crossreactivity: mouse and canine. Species sequence homology: rat.