Furin Convertase, Region 2

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10kD amino-terminal proregion, a highly conserved ~55kD subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface. \n\nApplications:\nSuitable for use in Immunocytochemistry, Immunoprecipitation, and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nImmunocytochemistry: 1:200-1:400\nImmunoprecipitation: 1:200 (50mM Tris/HCl, pH 7.4, 150mM sodium chloride, 1% Triton X-100; 1% sodium deoxycholate, 0.1% SDS)\nWestern Blot: 1:1000\nOptimal dilution to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9201-11

Size

100ul

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid, no preservatives added.

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

1. Development and characterization of a panel of monoclonal antibodies against the novel subtilisin-like proprotein processing enzyme furin: H.L. van Duijnhoven, et al.; Hybridoma 11, 71 (1992) 2. Modulation of furin-mediated proprotein processing activity by site-directed mutagenesis: J.W. Creemers, et al.; J. Biol. Chem. 268, 21826 (1993) 3. Structure and function of eukaryotic proprotein processing enzymes of the subtilisin family of serine proteases: W.J. Van de Ven, et al.; Crit. Rev. Oncog. 4, 115 (1993) 4. Polyarginines are potent furin inhibitors: A. Cameron, et al.; J. Biol. Chem. 275, 36741 (2000) 5. 'Shed' furin: mapping of the cleavage determinants and identification of its C-terminus: B. Plaimauer, et al.; Biochem. J. 354, 689 (2001)

Additional Info

Recognizes epitope region 2 (R2) in the catalytic domain of human furin convertase. Species crossreactivity: mouse.