Furin (Dibasic-processing Enzyme, PACE, Paired Basic Amino Acid Residue-cleaving Enzyme, FUR, PACE, PCSK3)

Furin, also known as Paired basic aa residue cleaving enzyme, is a 794aa containing membrane associated protein circulating between the trans-Golgi network and cell surface. It belongs to the peptidase S8 family and Furin subfamily with a homo B/P domain. Known to represent the ubiquitous endoprotease activity within constitutive secretory pathways, Furin, a calcium-dependent serine endoprotease, can efficiently cleave precursor proteins at their paired basic aa processing sites (RX (K/R) R consensus motif). This proprotein convertase enzyme processes latent precursor proteins into their biologically active products and is important in the release of albumin and complement component C3 from their respective precursors and in the physiologic processing of proparathyroid hormone, von Willebrand factor, insulin receptor, TGF1B, hepatocyte growth factor, C propeptide of collagen type V, alpha 1, NOTCH signaling proteins, certain viral envelope glycoproteins and ED1. Ubiquitous expression of Furin is seen in most of the tissues. It may also play a role in tumor progression and maintainance of immune tolerance.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9200-01A

Size

100ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Pesu, M. et al. Nature. 455:246-250 (2008). 2. Blanchette, F. et al. J. Clin. Invest. 99:1974-1983 (1997). 3. Hendy, GN. J. Biol. Chem. 270:9517-9525 (1995).

Additional Info

Recognizes human Furin. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, rat, rhesus monkey. Species sequence homology: chicken, opossum, xenopus.