Fyn, p59

Fyn is a 59kD non-receptor protein tyrosine kinase belonging to the Src family of cytoplasmic tyrosine kinases. Members of this family possess a similar structural organization which can be divided into four distinct domains including: 1) N-terminal myristoylation on glycine- required for membrane localization, 2) a unique N-terminal domain which may dictate specificity of association, 3) conserved SH3 and SH2 domains, and 4) a C-terminal catalytic domain(2). Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing(3). Fyn was also the first tyrosine kinase found to be associated with the T cell antigen receptor complex(4). The interaction of Fyn with the TCR complex is mediated by the amino terminal domain of Fyn and at least one ARAM sequence (antigen recognition activation motif) in the TCR zeta chain (TCRz)(2). In addition to its association with TCRz, Fyn has been found to associate with the p21ras interacting protein p62, and this interaction is dependent upon the SH3 domain of Fyn(5). Studies on mice expressing kinase-inactive Fyn and on Fyn knockout mice indicate that Fyn is likely to play a role in the later stages of thymocyte development, but is not required for TCR- mediated proliferation in peripheral cells(6,7,8).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nImmunoprecipitation, IP-Kinase Assay: 2-5ug \nWestern Blot: 1-3ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F9500-08

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Olszowy, M.W., et al; Journal of Immunology Manuscript in preparation (1995). 2. Gauen, L.K.T., et al; Mol. Cell. Biol 12:5438-5446 (1992). 3. Cooke, M.P., et al; New Biologist 1:66-74 (1989). 4. Samelson, L.E., et al; Proc. Natl. Acad. Sci. USA 87:4358-4362 (1990). 5. Richard, S., et al;. Mol. and Cell. Biol 15:186-197 (1995). 6. Appleby, M.W.., et al; Cell 70:751-763 (1992). 7. Cooke, M.P., et al; Cell 65:281-292 (1991). 8. Stein, P.L., et al; Cell 70:741-750 (1992). 9. Rudd, C.E., et al;Proc. Natl. Acad. Sci. USA 85:5190-5194 (1988). 10. Chan, A.C., et al; A. Ann. Rev. Immunol.12:555-592 (1994). 11. Cooper, J.A.;Peptides and Protein Phosphorylation, eds B Kemp, PF Alewood, CRC Press Boca Raton, Fl (1989).

Additional Info

Recognizes a unique region of p59 fyn (Fyn) between aa26-75 . Reactivity has been con?rmed by immunoprecipitation or western blot analysis of extracts derived from human T cell hybridomas, Jurkat cells, mouse T cell hybridomas and recombinant Fyn.