Fyn (Src Family Tyrosine Kinase)

Fyn is a member of the Src family of non-receptor protein tyrosine kinases. It is is highly expressed in brain. Fyn has been shown to be involved in T cell and B cell activation and keratinocyte differentiation. Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2 responsive cell line. In T cells, Fyn associates with the T cell antigen receptor and Thy-1. A study of human T-cells revealed that Lck and Fyn had different intracellular locations, suggesting that these two members of the Src family regulate different aspects of T cell activation. The Src homology 3 (SH3) region of Fyn can bind the proline-rich domain of the Fas ligand. In addition, Raf-1 may form a complex with Fyn through its Src Homology 2 (SH2) domain and serine phosphorylation of Raf-1. The N-terminal domain of p59fyn can bind other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of fyn activity. p59fyn is highly expressed in brain suggesting that it may play a role in the sensory nervous network and in myelination at early stages of CNS formation.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry, Western Blot and Kinase Assay. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen and formalin/paraffin sections): 1-2ug/ml for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. \nOptimal dilutions to be determined by the researcher.\n \nPositive Control: \nJurkat cells and lymphomas.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F9500-04

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

Additional Info

Recognizes a protein of 59kD which is identified as Fyn. Demonstrates no crossreactivity with other members of the Src protein kinase family. Epitope spans aa26-75 of the p59fyn region.\nSpecies Crossreactivity: Human, mouse and rat.