G Protein, alpha, CT (Goa)

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of a-subunits (ao1 and a2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C-terminal third of the deduced amino acid sequence. Some of the literature suggests that Transducin is the generic name for the heterotrimeric G protein or possibly Gt. Transducin is composed of several subunits. G proteins are further categorized into different classes, including Go and Gi, based on their aa sequence. Goa is a subunit of Go. Goa could be one subgroup of Transducin. However, there is some literature stating that Go, Gi, and Transducin are all separate groups of G proteins.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot 1:1000. Detects a single protein band of 39-42kD in bovine and rat membranes.\nOptimal dilutions to be determined by researcher.\n\nSource: Ascites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8599-03

Size

100ul

Applications

WB

Hosts

Mouse

Reactivities

Bov

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Li, X., Mumby, S., Greenwood, A., Jope, R., Pertussis Toxin-Sensitive G-Protein a-Subunits: Production of Monoclonal Antibodies and Detection of Differential Increases Upon Differentiation of PC12 and LA-N-5 Cells. J. Neurochem. 64: 1107 (1995). 2. Sternweis and Robishaw, J. Biol. Chem. 259: 13806 (1984). 3. Dhingra, A., et al., J. Neuroscience 22: 4878-4884 (1995). 4. Lynch, et al., J. Clin. Invest. 83: 2050-2062 (1989). 5. Gettys, et al., J. Biol. Chem. 266: 15,949-15,955 (1991). 6. Luthin, et al., J. Biol. Chem. 268(8): 5990-5996 (1993). 7. Caro, et al., J. Cell. Biochem. 54: 309-319 (1994). 7. Navon, S.E., Fung, B.K., Characterization of transducin from bovine retinal rod outer segments. Use of monoclonal antibodies to probe the structure and function of the subunit. J. Biol. Chem. 263(1): 489-96 (1988, Jan 5). 8. Halpern, J.L., Tsai, S.C., Adamik, R., Kanaho, Y., Bekesi, E., Kung, H.F., Moss, J., Vaughan, M., Structural and functional characterization of guanyl nucleotide-binding proteins using monoclonal antibodies to the alpha-subunit of transducin. Mol. Pharmacol. 29(5): 515-9 (1986, May). 9. Halpern, J.L., Moss, J., Immunological characterization of guanine nucleotide-binding proteins: effects of a monoclonal antibody against the gamma subunit of transducin on guanine nucleotide-binding protein-receptor interactions. Mol. Pharmacol. 37(6): 797-800 (1990, June).\n10. www.harvester.embl.de/harvester/P114/P11488.htm\n11. www.gpcr.org/7tm/seq/190_001/190_001.SEQ.html

Additional Info

Recognizes Goa. Species Crossreactivity: Strong crossreactivity with bovine (100%) and human (58%). Slight crossreactivity with guinea pig (24%) and rat (26%). Does not crossreact with mouse. Specificity confirmed by Western Blot versus bovine Gat and Gsa; rat Goa, Gia-1, Gia-2 and Gia-3; mouse GoaA and GoaB