G Protein alpha Inhibitor 1, 2 (Adenylate cyclase inhibiting G alpha protein, Gi, Gi1 protein alpha subunit, Gi2 protein alpha subunit, GIP, GNAI 1, GNAI 2, GNAI 2B, GNAI1, GNAI2, GNAI2 protein, GNAI2B, GTP binding regulatory protein Gi alpha 2 chain, Gua

The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8599-55B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

PBS, 1 mg/ml BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Sugden PH & Clerk A Activation of the small GTP-binding protein Ras in the heart by hypertrophic agonists. Trends Cardiovasc Med 10:1-8 (2000). <PUBMED:11150721>Wilkie TM et al. Evolution of the mammalian G protein alpha subunit multigene family. Nat Genet 1:85-91 (1992). <PUBMED:1302014>

Additional Info

Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with bovine, Chicken, canine, Guinea pig, Hamster, Starfish and Xenopus laevis due to sequence homology. Not yet tested in other species.

Alternative Names

Adenylate cyclase inhibiting G alpha protein, Gi, Gi1 protein alpha subunit, Gi2 protein alpha subunit, GIP, \nGNAI 1, GNAI 2, GNAI 2B, GNAI1, GNAI2, GNAI2 protein, GNAI2B, GTP binding regulatory protein Gi alpha 2 chain, Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1, Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 2, Guanine nucleotide binding protein alpha inhibiting activity polypeptide 1, Guanine nucleotide binding protein alpha inhibiting activity polypeptide 2, Guanine nucleotide binding protein G(i) alpha 1 subunit, Guanine nucleotide binding protein G(i) alpha 2 subunit, H LUCA15.1, H LUCA16.1