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G Protein Coupled Receptor 182 (GPR182, 7TMR, Adrenomedullin Receptor L1, ADMR, AMR, AM-R, G10D, Gamrh, hrhAMR, MGC34399)

Cat no: G8601-86

G Protein Coupled Receptor 182 (GPR182, 7TMR, Adrenomedullin Receptor L1, ADMR, AMR, AM-R, G10D, Gamrh, hrhAMR, MGC34399)

G Protein-Coupled Receptor L1/G10D (GPR182) has been suggested to be an Adrenomedullin Receptor. Originally cloned from rats and later from humans, L1/G10D does not to bind adrenomedullin or display functional cAMP response to adrenomedullin (reviewed in Poyner et al. 2002). The initial experimental results, which demonstrated high-affinity binding and increased levels of cAMP in COS cells that were transfected with L1/G10D and treated with adrenomedullin, could not be reproduced by other laboratories (Kapas et al. 1995). It is now known that the functional adrenomedullin receptor is a heterodimeric complex composed of calcitonin receptor-like receptor (CRLR) and receptor activity modifying proteins (RAMPs) (McLatchie et al. 1998). L1/G10D therefore should be considered an Orphan A receptor.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 6-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman testis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8601-86

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.7, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human GPR182.

Alternative Names

G Protein-coupled Receptor 182, G-protein Coupled Receptor 182

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