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G Protein Coupled Receptor 34 (GPCR34, GPR34)

Cat no: G8600-40

G Protein Coupled Receptor 34 (GPCR34, GPR34)

GPR34 is a widely expressed 75-90kD seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% aa sequence identity with mouse and rat GPR34.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 25ug/ml. Use 10ul to label 1-5x10e5 cells in a total volume (same/less than) 200ul. Validated using A172 cells fixed first with 4% paraformaldehyde and 0.1% saponin in PBS.\nImmunocytochemistry: 10ug/ml. A172 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. Between each step, cells were washed with PBS, BSA.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8600-40

Size

100ug

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes human GPR34. Stains human GPR34 transfectants but not irrelevant transfectants.

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