GPR34 is a widely expressed 75-90kD seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% aa sequence identity with mouse and rat GPR34.
Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 25ug/ml. Use 10ul to label 1-5x10e5 cells in a total volume (same/less than) 200ul. Validated using A172 cells fixed first with 4% paraformaldehyde and 0.1% saponin in PBS.
Immunocytochemistry: 10ug/ml. A172 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. Between each step, cells were washed with PBS, BSA.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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