GPR4 is a Lysophospholipid/Lysosphingolipid receptor. Recently, GPR4 has been shown to act as a proton-sensing receptor stimulating inositol phosphate formation (Ludwig et al. 2003). GPR4 has been reported in kidney, heart, and especially lung, where it is at least five-fold greater than in other tissues (Mahadevan et al., 1995). ESTs have been isolated from brain, colon, kidney, lung, and melanocyte/uterus/fetal heart libraries.
Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 24ug/ml
Immunofluorescence /ICC: 24ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Human transfection-cells expressing vector only
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.