G Protein, s alpha (Gsa, GNAS)

The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA (Peptide): 1:1000 \nOptimal dilutions to be determined by researcher.\n\nRecommended Secondary Antibodies: \nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G9005

Size

50ul

Applications

ELISA, ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Lim SH, Poh LK, Cowell CT, Tey BH, Loke KY: Mutational analysis of the GNAS1 exons encoding the stimulatory G protein in five patients with pseudohypoparathyroidism type 1a. J. Pediatr. Endocrinol. Metab. 15(3): 259-268 (2002). 2. Shore EM, Ahn J, Jan de Beur S, Li M, Xu M, Gardner RJ, Zasloff MA, Whyte MP, Levine MA, Kaplan FS: Paternally inherited inactivating mutations of the GNAS1 gene in progressive osseous heteroplasia. N. Engl. J. Med. 346 (2): 99-106 (2002). 3. Hayward BE, Moran V, Strain L, Bonthron DT: Bidirectional imprinting of a single gene: GNAS1 encodes maternally, paternally, and biallelically derived proteins. Proc. Natl. Acad. Sci. U S A. 95(26): 15475-15480 (1998). 4. Shenker A, Chanson P, Weinstein LS, Chi P, Spiegel AM, Lomri A, Marie PJ: Osteoblastic cells derived from isolated lesions of fibrous dysplasia contain activating somatic mutations of the Gs alpha gene. Hum. Mol. Genet. 4(9): 1675-1676 (1995). 5. Kehlenbach RH, Matthey J, Huttner WB: XL alpha s is a new type of G protein. Nature 372(6508): 804-809 (1994). 6. Miric A, Vechio JD, Levine MA.: Heterogeneous mutations in the gene encoding the alpha-subunit of the stimulatory G protein of adenylyl cyclase in Albright hereditary osteodystrophy. J. Clin. Endocrinol. Metab. 76(6): 1560-1568 (1993).

Additional Info

Recognizes both the large (MW ~52kD) and small (MW ~45kD) forms of Gs alpha. Does not crossreact with other G proteins. Species Crossreactivity: human and mouse

Alternative Names

GSA, GSP, POH, GPSA, NESP, GNAS1, GNASXL, NESP55, G-s-alpha neuroendocrine secretory protein 55, guanine nucleotide regulatory protein, paternally expressed G protein XL alpha S, adenylate cyclasestimulating G alpha protein, guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide