G3BP-1 (GTPase activating protein (SH3 domain) binding protein 1, ATP-dependent DNA helicase VIII, G3BP, G3BP-1, GAP SH3 domain-binding protein 1, HDH-VIII, MGC111040, Ras GTPase-activating protein-binding protein 1)

G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). It was first discovered by its binding to RasGAP. G3BPs have been shown to be involved in a number of mitogenic signaling pathways. G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007). Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G0875-01A

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.05% NaN3.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. White, J., et al. (2007) Cell Host & Microbe 2: 295-305. 2. Rivera, C. and R.E. Lloyd. (2008) Virology 375: 59-72. 3. Kim, M, et al. (2007) Oncogene 26; 4209-4215.

Additional Info

Recognizes human G3BP1 at its C-terminal region. Species Crossreactivity: mouse. Species sequence homology: rat (100%).