G9a (EHMT2, Euchromatic Histone-lysine N-methyltransferase 2)

G9a is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-di- merization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large tran- scriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and his- tone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12,13).\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nImmunofluorescence (IF-IC): 1:50\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G1009-02

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

(1) Tachibana, M. et al. (2001) J Biol Chem 276, 25309-17. (2) Patnaik, D. et al. (2004) J Biol Chem 279, 53248-58. (3) Tachibana, M. et al. (2002) Genes Dev 16, 1779-91. (4) Tachibana, M. et al. (2005) Genes Dev 19, 815-26. (5) Sampath, S.C. et al. (2007) Mol Cell 27, 596-608. (6) Ueda, J. et al. (2006) J Biol Chem 281, 20120-8. (7) Ogawa, H. et al. (2002) Science 296, 1132-6. (8) Shi, Y. et al. (2003) Nature 422, 735-8. (9) Nishio, H. and Walsh, M.J. (2004) Proc Natl Acad Sci USA 101, 11257-62. (10) Este?ve, P.O. et al. (2006) Genes Dev 20, 3089-103. (11) Tachibana, M. et al. (2007) EMBO J 26, 3346-59. (12) Kondo, Y. et al. (2007) Hepatol Res 37, 974-83

Additional Info

Recognizes endogenous levels of total human G9a (both the 165kD G9a-L and 140kD G9a-S isoforms). Does not crossreact with other histone methyl-transferases, including GLP. Species Crossreactivity: Mouse, rat, monkey. Species Sequence Homology: Porcine, bovine, equine.