Gab1, phosphorylated (Tyr627) (Grb2-Associated Binder1)

Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta (PDGFR beta), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met) and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma-1, Nck and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2 and for activation of the ERK/MAPK pathway in response to EGF.\n\nApplications: \nSuitable for use in ELISA and Western blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nNIH3T3 cells +/- PDGF. Gab1 (Grb2-associated binder protein-1) is a ~115kD multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1012-21

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA , pH 7.3, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Hogado-Madruga, M. and A.J. Wong Cancer Res. 64(6): 2007-2015.(2004). 2. Ren, Y., et al. J. Biol. Chem. 279(9): 8497-8505.(2004). 3. Hogado-Madruga, M. and A.J. Wong Mol. Cell. Biol. 23(13): 4471-4484.(2003) 4. Y. Wang Circ. Res. 93(3): 186-188.(2003) 5. Kim, S.O., et al. Endocrinology 143(12): 4856-4867.(2002) 6. Saito, Y., et al. . J. Biol.Chem. 277(26): 23216-23222.(2002) 7. Cunnick, J.M., et al. J. Biol. Chem.276(26): 24380-24387.(2001) 8. Shinohara, M., et al. J. Biol. Chem. 276(22): 18941-18946.(2001) 9. Lehr, S., et al. . Biochemistry 39(35): 10898-10907.(2000).

Additional Info

Recognizes Human Gab1 when phosphorylated at Tyr627. Species sequence homology: Mouse. Does not cross react with Gab1 phosphorylated at tyrosines 242, 437, 472 or 476.