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GABA A receptor beta 1 subunit (CT)

GABA A receptor beta 1 subunit (CT)

Cat no: G1015-06NA

Supplier: United States Biological
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GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of seven subunit types; alpha, beta, g, d, E, 0, and ?. Three subunits, alpha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta and g subunits. The rat beta 1 isoform is a 58-60 kD, 449aa, 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa13-218). The beta 1 subunit is known to be phosphorylated on a consensus phosphorylation site (S409 of the precursor) that exists in the cytoplasmic domain between transmembrane segments 3 and 4. PKA or PKC beta II-induced phosphorylation decreases GABA A receptor activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Sufficient for 10 mini-blots. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G1015-06NA
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607. 2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1. 3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119. 4. Hinkle, D.J. and R.L. MacDonald (2003) J. Neurosci. 23:11698. 5. Brandon, N.J. et al. (2003) Mol. Cell. Neurosci. 22:87.
Additional info: Recognizes rat GABA-A-R beta 1 at ~55kD. Species Cross Reactivity: human, and mouse.

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