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GABA A receptor beta 2 subunit (CT)

GABA A receptor beta 2 subunit (CT)

Cat no: G1016-10A

Supplier: United States Biological
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GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of seven subunit types; alpha, beta, g, d, E, 0, and ?. Three subunits, alpha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta and g subunits. The rat beta 2 isoform is a 55kD, 450aa, 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus aa13-218. The beta 2 subunit is part of the most common GABA A receptor combination in the mammalian brain (a1b2g2). Like the other two beta subunits, beta 2 is phosphorylated on a consensus phosphorylation site (S410 of the precursor) that exists in the second cytoplasmic domain. In contrast to the other beta subunits, only PKC is capable of this modification. However, tyrosine phosphorylation of beta 2 is known, and this apparently increases GABAA current amplitude. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Sufficient for 10 mini-blots. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G1016-10A
Reactivities: Human, Mouse, Rat, Bovine
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607. 2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1. 3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119. 4. Brandon, N.J. et al. (2002) J. Neurosci. 22:6353. 5. McDonald, B.J. et al. (1998) Nat. Neurosci. 1:23. 6. Wan, Q. et al. (1997) J. Neurosci. 17:5062.
Additional info: Recognizes rat GABA-A-R beta 2 at ~50-56kD. Species Cross Reactivity: bovine, human, and mouse.

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