GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GAA1)

GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain. GABA exerts its effects through ionotropic ligand-gated GABA-A, GABA-C and GABA-B receptors (GABAbetaRs). A family of GABA-A receptors subtypes exists. These receptors are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta and rho1-3 to form a heteromeric (pentameric) protein complexes. Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1). GABA A (GAA) receptor proteins (450-627aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM \n(transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain. The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits. This variability may confer subunit specific functionality. GAA genes are distributed as clusters throughout the human genome \n(chromosomes 4, 5, 15 and X; delta subunit on chromosome 1). GAA in the brain is the target of many clinically important drugs.\nHuman GAA1 (chromosome 5q34-q35) protein is 456aa \n(rat/mouse: 455aa). Defects in GABRA1 are a cause of Juvenile Myoclonic Epilepsy (JME), a common epileptic syndrome characterized by febrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL technique.\nELISA: 0.5-1ug/ml. Coat plates at 1ug/ml with control peptide.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nG1015-06H\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-06E

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird

Form

Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Mehta, A.K., et al., Brain Res. Rev. 29: 196-271 (1999). 2. Whiting, P.J., et al., Ann. NY Acad. Sci. 868: 645-653 (1999). 3. Siegart, W., et al., Neurochem. Intl. 34: 379-385 (1999). 4. Garrett, K.M., et al., BBRC 156: 1039-1045 (1988). 5. Lolait, S.J., et al., FEBS Lett. 246: 145-148 (1989). 6. Wang, J.B., et al., J. Mol. Neurosci. 3: 177-184 (1992). 7. Schofield, P.R., et al., FEBS Lett. 244: 361-364 (1989). 8. Hirouchi, M., et al., Neurochem. Intl. 15: 33-38 (1989). 9. Johnson, K.J., et al., Genomics 14: 745-748 (1992). 10. Kang, I., et al., J. Neurochem. 6: 1643-1646 (1994).

Additional Info

Recognizes human GAA1. No significant homology is detected with other GABA A receptor subtypes or other receptors. \nSpecies Crossreactivity: Mouse, rat, bovine and chicken.