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GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA)

GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA)

Cat no: G1015-09D

Supplier: United States Biological
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GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of seven subunit types; aplha, beta, g, d, E, 0, and ?. Three subunits, aplha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta, and g subunits. The rat alpha 1 isoform is a 50-52kD, 428aa, 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus aa15-222. As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the aplha 1 subunit and regulate benzodiazepine binding. Alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Sufficient for 10 mini-blots. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G1015-09D
Reactivities: Human, Mouse, Rat, Bovine
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607. 2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1. 3. Churn, S.B. et al. (2002) J. Neurochem. 82:1065. 4. Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408. 5. Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.
Additional info: Recognizes rat GABA A R alpha 1 at ~51kD. Species Cross Reactivity: bovine, human, and mouse.

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