GABA Receptor A, alpha 4, (GABA(A) receptor subunit alpha 4, GABR A4, GABRA4, Gamma aminobutyric acid (GABA) A receptor alpha 4)

GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family \nof neurotransmitter-gated ion channels. GABA binding to A-type receptors induces an- \nion-selective ion channel opening. These receptors are the principal fast inhibitory neuro- \ntransmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of \nseven subunit types; alpha, beta, g, d, E, 0, and ?. Three subunits, alpha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some \ncombination of alpha, beta and g subunits. The rat alpha 4 isoform is a 67kD, 517aa, \n4 transmembrane protein with two terminal extracellular regions. The ligand-binding re- \ngion is in the N-terminus aa14-221. The alpha 4 subunit is unusual in that it does not seem \nto preferentially form channel complexes with the g subunit. In hippocampal neurons, alpha \n4 subunits apparently form GABA receptors that do not cluster, but exist in diffuse net- \nworks in nonsynaptic membranes. \n \nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Sufficient for 10 mini-blots.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-20F

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607. 2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1. 3. Wisden, W. et al. (1991) FEBS Lett. 289:227. 4. Bencsits, E. et al. (1999) J. Biol. Chem. 274:19613. 5. Mangan, P.S. et al. (2005) Mol. Pharmacol. 67:775.

Additional Info

Specific for the ~64kD a 4 subunit of the GABAA Receptor in Western blots of rat brain lysates. Also recognizes the a 4 subunit of the GABAA Receptor in bovine, human and mouse brain lysates.