GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family
of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces
anion-selective ion channel opening. These receptors are the principal fast inhibitory
neurotransmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of seven subunit types; alpha, beta, g, d, E, 0, and ?. Three subunits, alpha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta and g subunits. The rat alpha 6 isoform is a 57kD, 434, 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa14-221). The expression of the subunit is strongly associated with cerebellar granule cells. The alpha 6 subunit has also been linked to alcohol sensitivity, possible due to a point mutation substitution of arginine for a glutamine at aa position 81 in the extracellular N-terminus.
Applications:
Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000. Sufficient for 10 mini-blots.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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