GABA Receptor A, gamma 2 (GAG2)

GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain. GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs). A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric) protein complexes. Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1). GABA A (GAA) receptor proteins (450-627 aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain. The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits, which may confer subunit specific functionality. GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15, and X; delta subunit on chromosome 1). GAA in the brain are the targets of many clinically important drugs.\n\nApplications: \nSuitable for use in ELISA, Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting : 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.\n

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SPECIFICATIONS

Catalog Number

G1015-20L

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

(1). Mehta Ak and Ticku MK et al (1999) Brain Res. Rev. 29, 196-271 (review); Whiting PJ et al (1999) Ann. NY Acad. Sci. 868, 645-653 (review); Siegart W et al (1999) Neurochem. Intl. 34, 379-385 (review).\n(2). Shivers BD et al (1989) Neuron 3, 327-337; Malherbe, P et al (1990) J. Neurosci. 10, 2330-2337; Jiang S et al (1999) Gene Accession #AF165124; Skela JM S et al (2000) Gene Accession #NM008073.

Additional Info

Recognizes GABA receptor A, Gama 2. The 30 AA rat control peptide is 100% conserved in bovine, human and mouse GAG2 (2). It is ~80% conserved in chicken GAB2. Significant homology is also seen with rat GAG1 (64%), ratGAG3 (55%). It is not clear if anti-GAG21 actually reacts with other gamma subunits. No significant homology is observed with other GAA subunits (alpha, beta etc.). Antibody crossreactivity of anti-GAG21 in various species has also not been studied.