GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family
of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neuro-
transmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of
seven subunit types; alpha, beta, g, d, E, 0, and ?. Three subunits, alpha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some
combination of alpha, beta and g subunits. The rat g2 isoform is a 48kD, 436aa, 4
transmembrane protein with two terminal extracellular regions. The ligand-binding region
is in the N-terminus aa30-233. The g 2 subunit is part of the most common GABAA
receptor combination in the mammalian brain (alpha 1 beta 2g2). GABA binds at alpha-beta interfaces, while benzodiazepine binds to alpha-g interfaces. There are two splice forms, the longest contains a consensus phosphorylation site in the second cytoplasmic domain, and a
short form that shows an absence of this site through a deletion of aa376-383. PKC
phosphorylates the long form at S381, while both the short and long forms are phos-
phorylated at S365. Phosphorylation blocks receptor activity. The g 2 subunits are also
palmitoylated at multiple sites on cysteines that lay between aa415-461, facilitating membrane trafficking.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000. Sufficient for 10 mini-blots.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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