GABA Receptor A, gamma 2 (Gamma-aminobutyric Acid Receptor Type A gamma Subunit, GABRA)

GABA A (g-aminobutyric acid-type A) receptors are members of the cysteine-loop family \nof neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neuro- \ntransmitter receptors in the CNS. GABA A receptors are heteropentamer combinations of \nseven subunit types; alpha, beta, g, d, E, 0, and ?. Three subunits, alpha, beta, and g, have at least three separate gene products in mammals, and typical GABA A receptors have some \ncombination of alpha, beta and g subunits. The rat g2 isoform is a 48kD, 436aa, 4 \ntransmembrane protein with two terminal extracellular regions. The ligand-binding region \nis in the N-terminus aa30-233. The g 2 subunit is part of the most common GABAA \nreceptor combination in the mammalian brain (alpha 1 beta 2g2). GABA binds at alpha-beta interfaces, while benzodiazepine binds to alpha-g interfaces. There are two splice forms, the longest contains a consensus phosphorylation site in the second cytoplasmic domain, and a \nshort form that shows an absence of this site through a deletion of aa376-383. PKC \nphosphorylates the long form at S381, while both the short and long forms are phos- \nphorylated at S365. Phosphorylation blocks receptor activity. The g 2 subunits are also \npalmitoylated at multiple sites on cysteines that lay between aa415-461, facilitating membrane trafficking. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Sufficient for 10 mini-blots.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-20H

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607. 2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1. 3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119. 4. Krishek, B.J. et al. (1994) Neuron 12:1081. 5. Keller, C.A. et al. (2004) J. Neurosci. 24:5881. 6. Moss, S.J. et al. (1992) J. Biol. Chem. 267:14470.

Additional Info

Recognizes rat GABA-A-R gamma 2 at ~44-47kD. Species Cross Reactivity: bovine, human, and mouse.