GABA Receptor B 1b (GBR1b)

GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain. GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs). Presynaptic GABABRs inhibit neurotransmitter release by down regulating high voltage activated Ca+2 channels, whereas postsynaptic GABABRs decrease neuronal excitability by activating a prominent inwardly rectifying K+ (Kir) conductance that underlies the late inhibitory postsynaptic potential. Two N-terminal splice variants GABABR1 (also called GBR1), GABABR1a (GBR1a, rat 960aa, apparent mol wt ~130kD; human 961aa) and GABABR1b (GBR1b; rat 844aa, apparent MW 100kD; human 844aa) have been cloned and characterized. GABABRs are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 7 TM domains, and C-terminal, cytoplasmic domain. GABABRs are expressed in all major areas of the brain. Recently GABABR2 (or GBR2) has been cloned from rat (940aa) and human (941aa). GBR2 forms heterodimer with GBR1 through interaction at the intracellular C-terminal regions. Heterodimerization appears to be important in transporting GBR1 to the membrane and expression of functional receptors. GBR2 also co-localized with GBR1.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL)\nELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nG1015-44D: GABA Receptor B 1b, Rat, Control Peptide (GBR1b)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nAlso Available:\nG1015-44: GABA Receptor B 1b (GBR1b) Pab Rb xRt\nG1015-44A: GABA Receptor B 1b (GBR1b) Pab Rb xRt

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SPECIFICATIONS

Catalog Number

G1015-44A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as liquid in PBS, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Kaupmann, K., et al., Nature 386: 239 (1997). 2. Kaupmann, K., et al., PNAS 95: 14991 (1998). 3. Kaupmann, K., et al., Nature 396: 683 (1998). 4. White, J.H., et al., Nature 396: 679 (1998). 5. Jones, K.A., et al., Nature 396: 674 (1998). 6. Kuner, R., et al., Science 283: 74 (1999).

Additional Info

Recognizes rat GBR1b. Peptide sequence is 100% conserved in various alternatively spliced rat GBR1b (844aa), GBR1c (875aa) and GBR1d (812aa). No significant sequence homology is detected with GBR1a or GBR2 or other GABA receptors. Species Sequence Homology: mouse: 100%; human: 94%.