GABA Receptor B R2 (Gamma-aminobutyric Acid Receptor Type B)

Strong GABAB-R2 receptor immunoreactivity is demonstrated in the Purkinje cells of the cerebellum, in the cerebral cortex, in the medial habelulae, in the spinal cord and in the brainstem. In individual Purkinje neurons, the immunoreactivity is preferentially observed in the periphery of the cells, most likely corresponding to the plasma membrane. Immunoreactivity is also in the entire dendritic tree extending into the molecular cell layer of the cerebellum.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:500-1:1000 using a Cy3 labeled secondary antibody. Enzymatic detection may be used. Substantially higher primary antibody dilutions will be necessary. Light 4% PFA fixation is recommended. Permeabilization with 0.1-0.2% Triton in the block only. \nNote: Not suitable for use in traditional formalin fixation.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-50

Size

50ul

Applications

IHC

Reactivities

Rat

Form

Supplied as a liquid, before the addition of glycerol to 40%. No preservative added.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kuhn, S.A., et al., Mol. Cell Neurosci. 25(2): 312-322 (2004). 2. Waldvogel, H.J., et al., J. Comp. Neurol. 470: 339-356 (2004). 3. Kuhn, S.A., et al., Molecular and Cellular Neuroscience 25: 1470-1480 (2004). 4. Panek, I., et al., Dept of Physiology and Biophysics, University Halifax, Nova Scotia and Dept of Biophysics, N. Copernicus University, Torun, Poland: THE DISTRIBUTION AND FUNCTION OF METABOTROBIC GABA RECEPTORS IN THE SPIDER PERIPHERAL MECHANOSENSILLA. Poster, Goettingen (2003). 5. Widmer, A., et al., Neuroscience 269: 3 (2003). 6. Leite-Morris, K.A., et al., Neurosci. Letters 317: 119-122 (2002). 7. Bowery, N.G., et al., J. Pharmacol. Exp. Ther. 292: 2-7 (2000). 8. Durkin, M.M., et al., Brain Res. Mol. Brain Res. 71: 185-200 (1999). 9. Jones, K.A., et al., Nature 396: 674-679 (1998). 10. Kaupmann, K., et al., Nature 396: 683-687 (1998). 11. Martin, S.C., et al., Mol. Cell. Neurosci. 13: 180-191 (1999).

Additional Info

Recognizes GABAB-R2 receptor. Stains nerve fibers and cell bodies in untreated animals. The staining pattern for the immunoreactive cell bodies obtained with G1015-50 corresponds to the pattern using in situ hybridization with probes to rat GABAB-R2 receptor mRNA. Species Crossreactivity: Rat and spider. Species sequence homology: human