GABA Transporter 2 (GAT-2 (Gamma aminobutyrIc acid)

GABA is a major inhibitory neurotransmitter and the GABAergic\ntransmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOHtermini\nare predicted to be intracellular. Two of the high affinity\n(Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity). GAT1and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000. \nELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nG1015-72: GABA Transporter 2, Rat Control Peptide (GAT-2, Rat (Gamma aminobutyrIc acid)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-71

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Liu, Q. R. et al (1993) J. Biol. Chem. 268:2106-2112; Borden, L. A. et al (1992) J. Biol. Chem. 267:21098-21104. 2. Ikegaki, N. et al (1994) Mol. Brain Res. 26:37-46

Additional Info

Recognizes rat GABA Transporter 2. No significant homology to any other known eukaryotic proteins. Species sequence homology: mouse,100%.