GABA Transporter 3 (GAT-3 (Gamma aminobutyrIc acid)

GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOH-termini are predicted to be intracellular. Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity). GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using Chemiluminescence technique. We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.\nELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml. \nImmunohistochemistry: 2-10ug/ml in paraformaldehyde fixed sections of tissues. \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nG1015-78 GABA Transporter 3, Rat Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-77

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Liu, Q. R. et al (1993) J. Biol. Chem. 268:2106-2112; 2. Clark, J. A. et al (1992) Neuron 9:337-348. 3. Borden, L. A. et al (1994) Recept. Channels 2: 207-213 4. Ikegaki, N. et al (1994) Mol. Brain Res. 26:37-46

Additional Info

Recognizes rat GAT3 without significant homology to any other known eukaryotic protein. Species sequence homology: Mouse-100%, human-92%.