GABARAP (GABAA Receptor Associated Protein)

GABAA receptor associated protein (GABARAP), which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane (1), is part of the Atg8 family with a key role in autophagy. Proteins in this family, including micotubulin light chain 3 (LC3) and GATE-16, become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagsome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137436

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Wang, H. et al. (1999) Nature 397, 69-72. 2. Shpilka, T. et al. (2011) Genome Biol 12, 226. 3. Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12. 4. Sou, Y.S. et al. (2006) J Biol Chem 281, 3017-24. 5. Tanida, I. et al. (2004) J Biol Chem 279, 36268-76. 6. Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50. 7. Tanida, I. et al. (2001) J Biol Chem 276, 1701-6. 8. Tanida, I. et al. (2002) J Biol Chem 277, 13739-44. 9. Chakrama, F.Z. et al. (2010) Autophagy 6, 495-505. 10. Pellerin, I. et al. (1993) Mol Cell Endocrinol 90, R17-21. 11. Vernier-Magnin, S. et al. (2001) Biochem Biophys Res

Additional Info

Recognizes endogenous levels of total human GABARAP. Species Crossreactivity: mouse, rat. An unknown background band is detected at 70kD. Does not cross-react with other GABARAP family members.