GADD45A (Growth arrest and DNA-damage-inducible protein GADD45 alpha, DNA-damage-inducible transcript 1, DDIT-1, DDIT1, GADD45)

GADD45 alpha, beta and epsilon are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1017-19B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and (same/less than)0.02% sodium azide.

P Type

Mab

Isotype

IgG

References

1. Wang, X.W. et al. (1999) Proc Natl Acad Sci USA 96, 3706-11, 2. Yang, Q. et al. (2000) J Biol Chem 275, 36892-8, 3. Zhao, H. et al. (2000) Exp Cell Res 258, 92-100, 4. Liebermann, D.A. and Hoffman, B. (2008) J Mol Signal 3, 15.

Additional Info

Recognizes human GADD45 alpha. Species crossreactivity: mouse, rat, monkey.