Galanin

Galanin is a 29aa C-terminally amidated (30 aa, non-amidated in humans), highly conserved but unique neuroendocrine peptide originally isolated from intestine. The first 14aa are fully conserved in almost all species. Galanin is found in the brain and the gut. It modulates a variety of physiological processed including cognition/memory, sensory/pain processing, neurotransmitter/hormone secretion, and feeding behavior. Several N-terminally elongated (-7-29 and -9-29) or truncated biologically active forms of galanin have also been isolated. Galanin antagonists are chimeric peptides generated by linking the amino terminal portion of galanin to substance P (galantide, M15), bradykinin (M35), the neurokinin antagonist spantide (C7) or an idealized alpha helical region (M40) \nGalanin mediated its biological effects by interacting with high affinity cell surface receptors. Pharmacological studies with several peptide antagonists and antagonists of galanin receptor suggest the existence of multiple receptor subtypes, and three galanin receptors (GAL-R1, GAL-R2, and GAL-R3) have been clone thus far. These receptors belong to the family of G-protein coupled receptor superfamily, characterized by seven transmembrane domains.\n\nApplications: \nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA:1:10,000-1:100,000, using 50-100ng of G1043-01D control peptide/well\nWestern Blot: 1-10ug/ml (ECL)\nImmunohistochemistry: 10-30ug/ml in formaldehyde fixed, paraffin-embedded tissues.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G1043-01A

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA. Also available without BSA. See G1043-01A1

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tatemoto K et al (1983) FEBS Lett. 164:124-128. 2. Vrontakis ME et al (1987) J Biol. Chem. 272:16755-16758. 3. Rokaeus A and Carlquist M (1988) FEBS Lett. 234:400-406. 4. Bersani et al (1991) FEBS Lett. 283:189-194. 5. Sillard R et al (1991) Peptides 12:855-859. 6. Wang Y and Conlon JM (1994) Peptides 15:981-986. 7. Melander T et al (1986) J Comp. Neurol. 248:475-517. 8. Melander T et al (1986) Neuroscience 19:223-240. 9. Skofitch G and Jacobwitz DM et al (1986) Peptides 6:509-546. 10. ibid (1986) Peptides 7:609-613. 11. Kask K et al (1995) Cell Mol Neurobiol. 15:653-674 (review). Zhao C-M, 2007 Endocrinologyin press, IHC.

Additional Info

Recognizes human galanin. No significant homology with other neuropeptide.