Galectin-1 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse GALECTIN-1 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nGalectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15kD.\n\nAssay Range:\n156pg/ml-10000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP16045\n\nEntrez Gene ID:\n16852\n\nKit Components:\n96-well plate pre-coated with anti-Galectin-1, Mouse\nLyophilized recombinant Galectin-1, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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