Galectin-1 (GAL1, LGALS1, GAL-1, Lectin Galactoside-binding Soluble 1, Beta-galactoside-binding Lectin L-14-I, Lactose-binding Lectin 1, S-Lac Lectin 1, Galaptin, 14kD Laminin Binding Protein, 14kD Lectin, HLBP14, HPL, HBL, Putative MAPK-activating Protei

Galectins are a family of b-galactose binding proteins that are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and their carbohydrate-binding site (1,2). Members of the Galectin family have been implicated in a variety of biological functions\nincluding cell adhesion (3), growth regulation (4), cytokine production (5), T-cell apoptosis (6), and immune responses (7). Galectin-1 (LGALS1) has been shown to be expressed in a wide range of tissues and cell types. The level and pattern of expression of LGALS1 has been\nshown to change during development (8). In addition to its apparent role in developmental processes, LGALS1 has been shown to be involved in central immune tolerance and may function in tumorigenesis by modulating the immune response to the tumor (9,10). Increased LGALS1 expression has been demonstrated in several human cancers, suggesting a correlation with metastatic potential (10).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G1044-47L

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Barondes, S.H. et al. (1994) Cell 76, 597-8. (2) Barondes, S.H. et al. (1994) J Biol Chem 269,\n20807-10. (3) Offner, H. et al. (1990) J Neuroimmunol 28, 177-84. (4) Wells, V. and Mallucci, L. (1991) Cell 64, 91-7. (5) Filer, A. et al. (2009) Arthritis Rheum 60, 1604-14. (6) Perillo, N.L. et al. (1995) Nature 378, 736-9. (7) Cooper, D.N. et al. (1991) J Cell Biol 115, 1437-48. (8) Puche, A.C. et al. (1996) Dev Biol 179, 274-87. (9) van den Br

Additional Info

Recognizes endogenous levels of total LGALS1. Species Crossreactivity: monkey, mouse, rat.