Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13)

Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20.\nDirect ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G1044-71

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

P Type

Mab

Purity

Purified by Protein G immunoaffinity chromatography.

Isotype

IgG2b

Additional Info

Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.