Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein, Lectin Galactose Binding Soluble 3, LGALS3, MAC2)

Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Among its numerous proposed functions, Galectin 3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin 3 shares an NWGR motif with Bcl-2 which has been shown to be an imjportant region in Bcl-2 for suppression of apoptosis. Galectin 3 has been shown to interact with Bcl-2 which may account for the ability of Galectin 3 to suppress programmed cell death in anti-Fas treated Jurkat cells transfected with Galectin 3. In contrast to Galectin 3, Galectin 1 actually induces apoptosis when added to cultures of activated human T cells. \n\nApplications:\nSuitable for use in Immunohistochemistry, Western Blot, ELISA, Flow Cytometry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:100-1:2000\nImmunohistochemistry: 1:10-1:2000\nImmunohistochemistry: (Paraffin): 6ug/ml \nWestern Blot: 1ug/ml. Detects a ~30kD protein representing Galactin 3 from Jurkat cells transfected with human Galactin 3.\nImmunocytochemistry(Immunofluorescence): 1:10-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRat Olfactory bulb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G1044-51

Size

100ug

Applications

ELISA, FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Biochemistry, 35: 6073-6079, 1996 2.:Proc. Natl. Acad. Sci., 93: 6737-6742, 1996 3. Biochemistry, 35: 6073-6079, 1996.

Additional Info

Recognizes human Galectin 3 at ~30kD. Species Crossreactivity: rat and mouse. Immunohistochemistry staining of Galectin 3 in rat olfactory bulb yields a pattern consistant with nuclear and plasma membrane staining.