GALNT10, NT (Polypeptide N-acetylgalactosaminyltransferase 10, Polypeptide GalNAc Transferase 10, GalNAc-T10, pp-GaNTase 10, Protein-UDP Acetylgalactosaminyltransferase 10, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10)

Protein glycosylation is an important biological process that is carried out by a large family of glycosyltransferases that catalyze the synthesis of oligosaccharides and glycoconjugates. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Polypeptide galactoaminyltransferase 10 (GALNT10) belongs to the polypeptide N-acetylgalactosaminyl-transferase (pp-GalNAc-T) protein family. Following expression in insect cells, recombinant GALNT10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both non-glycosylated and glycosylated peptide substrates. GALNT10 mRNA is highly expressed in several distinct hypothalamic, thalamic, and amygdaloid nuclei in mouse brain. At least four isoforms of GALNT10 are known to exist.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat Brain Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146487

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Amado M, Almeida R, Schwientek T, et al. Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim. Biophys. Acta 1999; 1473:35-53. 2. Nelson PA, Sutcliffe JG, and Thomas EA. A new UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase mRNA exhibits predominant expression in the hypothalamus, thalamus and amygdala for mouse forebrain. Gene Express. Patterns 2002; 1:95-9.

Additional Info

Recognizes human GALNT10. Species Crossreactivity: mouse and rat.