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Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)

Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)

Cat no: G2002-01

Supplier: United States Biological
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Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyzes the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 2.5-5ug/ml for 2 hours at RT. Immunoprecipitation: 10ug/mg protein lysate. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat, MAD109, PC12 cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G2002-01
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 500ul
Form: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Liu R M, et al. (1998) Am J Physiol: Lung Cell Molec Physiol, 275:L861-869. 2. Choi J, et al. (2000) J Biol Chem, 275:3693-3698. 3. Levonen A-L, et al. (2001) Arterioscler Thromb Vasc Biol, 21:1846-1851. 4. Moellering D, et al. (1999) FEBS Letts 448:292-296.
Additional info: Recognizes the heavy subunit of rat Gamma Glutamylcysteine Synthetase at ~73kD. Species Crossreactivity: human, mouse, monkey and bovine (weak).

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