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GAPDH (Glyceraldehyde 3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12)

GAPDH (Glyceraldehyde 3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12)

Cat no: G8140-16V

Supplier: United States Biological
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37kD subunits. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:10,000 Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8140-16V
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Baxi, M. D. and Vishwanatha, J. K. (1995) Biochemistry. 34, 9700-9707. 2. McNulty, S. E. and Toscano, W. A. Jr. (1995) Biochem. Biophys. Res. Commun. 34, 165-171. 3. Singh, R. and Green, M. R. (1993) Science. 259, 365-368. 4. Zang, W. Q. et al. (1998) Virology. 28, 46-52. 5. Han, X. et al. (1998) Biochem. Biophys. Acta. 1414,95-107. 6. Kragten, E. et al. (1998) J. Biol. Chem. 273, 5821-5828. 7. Koshy, B. (1996) Hum. Mol. Genet. 5, 1311-1318.
Additional info: Recognizes human GAPDH. Species Crossreactivity: mouse and rat.

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