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GAPDH (Glyceraldehyde 3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12) (HRP)

Cat no: G8140-13H

GAPDH (Glyceraldehyde 3-phosphate Dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12) (HRP)

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

G8140-13H

Size

50ug

Applications

IHC, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Goat, Prc, Rabbit

Form

Supplied as a liquid in PBS, pH7.4, 1% BSA, 0.01% thimerosal, 30% glycerol. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human GAPDH. Species Crossreactivity: Mouse, rat, goat, rabbit, chicken, hamster, bovine, porcine, fish, and E. coli.

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