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GAPDH, Loading Control (Glyceraldehyde 3-Phosphate Dehydrogenase)

GAPDH, Loading Control (Glyceraldehyde 3-Phosphate Dehydrogenase)

Cat no: G8140-15H

Supplier: United States Biological
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Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) is one of the key enzymes involved in glycolysis; it catalyzes the reversible oxidative phosphorylation of glyceraldehydes-3-phosphate. The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a "housekeeping" gene. Housekeeping proteins like GAPDH are useful as loading controls for western blots and protein normalization. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: >1:16,000 Western Blot: 0.01-0.1ug/ml Immunohistochemistry (paraffin): 0.3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Brain, heart, kidney, liver, lung, stomach, spleen, ovary, testis, and 293. Most cell lines and tissues Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8140-15H
Reactivities: Human, Mouse, Rat, Bovine, Canine, Drosophila/Arthropod, Non-Human Primate, Porcine
Hosts: Goat
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM,Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nat Med. 1996 Mar;2(3):347-50. PMID: 8612237. 2. Fortun, J., W.A. Dunn, S. Joy, J. Li, and L. Notterpek. 2003. Emerging role for autophagy in the removal of aggresomes in Schwann Cells. J. Neurosci. 23: 10672-10680. 3. Ellis,R.C., J.N. Earnhardt, R.L. Hayes, K.K. Wang, and D.K. Anderson. 2004. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J. Nuerochem. 88:689-697. 4. Kiepe D. et al.: Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I. Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12. PMID: 18556354.
Additional info: Recognizes GAPDH. Species Crossreactivity: bovine, canine, drosophila, human, monkey, mouse, porcine, rat. Species sequence homology: xenopus.

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