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Gata-1a, ID (gata1a, Zg1, gata1, zg1)

Gata-1a, ID (gata1a, Zg1, gata1, zg1)

Cat no: G2021-31

Supplier: United States Biological
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The GATA family of erythroid transcription factors is categorized by the ability to bind to the consensus sequence GATA. GATA transcription factors play important roles in the development of many organ systems, including hematopoietic, cardiovascular, reproductive, and gut-endoderm derived tissue. GATA transcription factor homologs in zebrafish are highly conserved in sequence, expression pattern, and function, making the zebrafish an appropriate model for the study of GATA factors. An in vivo study demonstrates the integral role of Gata-1 to erythroid differentiation: loss of Gata-1 alone results in the transformation of erythroid precursors into myeloid cells. Gata-1 expression undergoes positive autoregulation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Control Peptide: G2021-31P Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G2021-31
Reactivities: Zebrafish/Fish
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 250ul
Form: Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Heicklen-Klein, A et al (2005) Sem. Cell Dev. Biol. 16(1):95-106. 2. Galloway, JL et al (2005) Dev. Cell 8(1): 109-116. 3. Kobayashi, M et al (2001) Development 128(12): 2341-2350.
Additional info: Recognizes zebrafish Gata-1a.

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