GATA4, phosphorylated (Ser105) (GATA Binding Factor 4, GATA Binding Protein 4)

GATA-4 is a 46kD member of the GATA family of zinc finger-containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling; it plays a critical role in regulating basal and agonist or stress-induced gene expression in cardiac and smooth muscle cell types. GATA-4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA-4 is phosphorylated in response to agonist stimulation through MEK1-ERK1/2 and weakly through JNK or p38 MAPKs.\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher. \n\nPositive Controls Used: \nPhenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA-4.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G2021-51

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in Dulbecco

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Kitta, K., et al. (2002) Hepatocyte growth factor induces GATA-4 phosphorylation and cell survival in cardiac muscle cells. J. Biol. Chem. Dec. 4 [epub ahead of print]. 2. Clement, S.A., et al. (2002) Roles of protein kinase C and alpha-tocopherol in regulation of signal transduction for GATA-4 phosphorylation in HL-1 cardiac muscle cells. Free Radic. Biol. Med. 32(4):341-349. 3. Liang, Q., et al. (2001) The transcription factor GATA-4 is activated by extracellular signalregulated kinase 1- and 2- mediated phosphorylation of serine 105 in cardiomyocytes. Mol. Cell. Biol. 21(21):7460-7469. 4. Morisco, C., et al. (2001) Glycogen synthase kinase 3beta regulates GATA-4 in cardiac myocytes. J. Biol. Chem. 276(30):28586-28597.

Additional Info

Recognizes mouse GATA-4 when phosphorylated at Ser105. Species sequence homology: human, rat and chicken (100%).