GATM, Recombinant, Human, aa38-423, His-Tag (AGAT, Glycine Amidinotransferase, Mitochondrial, L-arginine:glycine Amidinotransferase, Transamidinase)

Supplier:

United States Biological

Cat no: 045429

GATM, Recombinant, Human, aa38-423, His-Tag (AGAT, Glycine Amidinotransferase, Mitochondrial, L-arginine:glycine Amidinotransferase, Transamidinase)

GATM (Glycine amidinotransferase, mitochondrial) is a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. It plays a role in embryonic and central nervous system development.\n\nSource:\nRecombinant corresponding to aa38-423 from human GATM, fused to His-tag at N-terminus, expressed in E.coli.\n\nMolecular Weight:\n~46.9kD (410aa) confirmed by MALDI-TOF\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMSTQAAT ASSRNSCAAD DKATEPLPKD CPVSSYNEWD PLEEVIVGRA ENACVPPFTI EVKANTYEKY WPFYQKQGGH YFPKDHLKKA VAEIEEMCNI LKTEGVTVRR PDPIDWSLKY KTPDFESTGL YSAMPRDILI VVGNEIIEAP MAWRSRFFEY RAYRSIIKDY FHRGAKWTTA PKPTMADELY NQDYPIHSVE DRHKLAAQGK FVTTEFEPCF DAADFIRAGR DIFAQRSQVT NYLGIEWMRR HLAPDYRVHI ISFKDPNPMH IDATFNIIGP GIVLSNPDRP CHQIDLFKKA GWTIITPPTP IIPDDHPLWM SSKWLSMNVL MLDEKRVMVD ANEVPIQKMF EKLGITTIKV NIRNANSLGG GFHCWTCDVR RRGTLQSYLD\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

045429

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 200mM sodium chloride, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Schulze A (2003). Mol. Cell. Biochem. 244 (1-2): 143-50. 2. Cullen M.E., Et al. (2006) Circulation 114:I16-I20.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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