B-Bridge GC HS 2x Master Mix I is an all-in-one 2x master mix containing HS DNA polymerase, GC Buffer I, enhancer, dNTPs and MgCl2. Simply mix GC HS 2x Master Mix I with primers, template and water and you are ready to carry out successful primer extensions. HS DNA Polymerase is a modified form of B-Bridge Taq DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases. Our GC-Rich DNA Target Kit and GC-Rich Master Mixes feature: high success rate of GC-rich DNA amplification, high specificity, sensitivity and product yield, diminished formation of non-specific product, and reaction set-up at room temperature. When PCR fails with regular Hot Start Master Mixes and buffers, due to high GC content, then try GC Buffer I either as a master mix or a kit for better results. If your amplification is still not satisfactory, then switch to GC Buffer II. GC buffer I and II successfully amplify targets with high GC percentage when regular buffers fall short.
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