GCSH, Recombinant, Human, aa49-173, His-tag (Glycine Cleavage System H Protein, Mitochondrial)

Supplier:

United States Biological

Cat no: 029658

GCSH, Recombinant, Human, aa49-173, His-tag (Glycine Cleavage System H Protein, Mitochondrial)

The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).\n\nSource:\nRecombinant corresponding to aa49-173 from human GCSH, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE\n\nMolecular Weight:\n~16.4kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

029658

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1mM DTT, 0.15M sodium chloride.

Purity

~95% (SDS-PAGE)

References

1. Kure S., et al. (2001) J Hum Genet. 46(7):378-84. 2. Kovata H., et al. (1991) 3. Am J Hum Genet. 48(2):351-61.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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