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GCTM-5

Cat no: G2023-60


Supplier: United States Biological
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The GCTM-5 monoclonal antibody reacts with a minority sub-population of cells in spontaneously differentiating cultures of pluripotent human embryonic stem cells and embryonal carcinoma. The epitope recognized by GCTM-5 is found on a 50kD protein present on the surface of these cells. Previous studies suggest that GCTM-5 may also prove to be a useful tool for defining cell lineage relationships between putative progenitor populations in embryonic liver and biliary epithelium during tissue repair (Pera M, et al, 2005). Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: 5-20ug/ml Western Blot: 0.5-2.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G2023-60
Reactivities: Human
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Stamp L., Pera M, et al., (2005). Stem Cells. 23(1):103-112. 2. Tosh D, et al., (2005). Hepatology. 42(1):S75-S84. 3. Costa M, et al., (2005). Nat Methods. Apr 2, 2(4):259-260. 4. Laslett A, et al., (2007). BMC Dev Biol. Mar 2, 7(1):12. 5. Andrews P, et al., (2007). Nat Biotechnol. Jun 17, 25(7):803-816.
Additional info: Recognizes human GCTM-5 antigen. The epitope recognized by GCTM-5 is found on a 50kD protein present on the surface of these cells.

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