GDF-8, BioAssay(TM) ELISA Kit (Myostatin, MSTN, Growth/Differentiation Factors, GDF-1 to GDF-15)

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems. \n\nIntended Use:\nFor the quantitative determination of Growth Differentiation Factor 8 (GDF-8) concentrations in cell culture supernates, tissue homogenates, serum, and plasma.\n\nSensitivity:\n5.32pg/ml\n\nRange:\n~31.3-2000pg/ml\n\nTest Principle:\nThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mature GDF-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any GDF-8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for mature GDF-8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDF-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.\n\nKit Components:\nMicroplate, 1x96wells\nConjugate (HRP), 1x21ml\nStandard, 1x20ng\nAssay Diluent, 1x11ml\nCalibrator Diluent concentrate, 1x21ml \nWash Buffer concentrate, 1x21ml\nColor Reagent A, 1x12ml\nColor Reagent B, 1x12ml\nStop Solution, 1x6ml\nPlate Sealers, 1x4 adhesive strips\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145740

Size

1Kit

Applications

ELISA

References

1. Lee, S.J. et al. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:183. 2. McPherron, A.C. et al. (1997) Nature 387:83. 3. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15842. 4. McFarlane, C. et al. (2005) Dev. Biol. 283:58. 5. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306. 6. Zimmers, T.A. et al. (2002) Science 296:1486. 7. Thies, R.S. et al. (2001) Growth Factors 18:251. 8. Amthor, H. et al. (2004) Dev. Biol. 270:19. 9. Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735. 10. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675. 11. Hill, J.J. et al. (2003) Mol. Endocrinol. 17:1144. 12. Kondas, K. et al. (2008) J. Biol. Chem. 283:23677. 13. Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027. 14. Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489. 15. Yang, J. et al. (2001) Mol. Reprod. Dev. 60:351. 16. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230. 17. Sharma, M. et al. (1999) J. Cell. Physiol. 180:1. 18. Heineke, J. et al. (2010) Circulation 121:419. 19. Breitbart, A. et al. (2011) Am. J. Physiol. Heart Circ. Physiol. 300:H1973. 20. Zhang, C. et al. (2012) Diabetologia 55:183. 21. Guo, T. et al. (2009) PloS ONE 4:e4937. 22. Zhang, C. et al. (2011) Diabetologia 54:1491. 23. McPherron, A.C. and S.J. Lee (2002) J. Clin. Invest. 109:595. 24. LeBrasseur, N.K. (2012) Diabetologia 55:13. 25. McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.